nusG [2020-10-02 16:07:38]

transcription antitermination and pausing factor

locus: BSU_01010

pI: 5.25

mw: 19.98 kDa

protein length: 177 aa

gene length: 534 bp

function: transcription

product: sequence-specific RNA polymerase pause factor

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.3|Transcription elongation/ termination]

Gene

Coordinates: 117,890 118,423

The protein

Catalyzed reaction/ biological activity

• binds to the non-template DNA within the paused transcription bubble [Pubmed|26742846]
• shifts [SW|RNA polymerase] to the posttranslocation register and induces pausing at 1,600 sites containing a consensus TTNTTT motif in the nontemplate DNA strand within the paused transcription bubble [pubmed|32817529]

[SW|Domains]

• KOW domain (aa 128-156) (according to UniProt)

Structure

• [PDB|2JVV] (from ''E. coli'', 44% identity) [Pubmed|19500594]

[SW|Localization]

• cytoplasm (according to UniProt)

Biological materials

Mutant

• GP2633 ([gene|43CFA3477B4EEB4CE0B2DF63ED2B5EDB171E79C7|nusG]::''cat''), available in [SW|Jörg Stülke]'s lab
• BKE01010 ([gene|43CFA3477B4EEB4CE0B2DF63ED2B5EDB171E79C7|nusG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTATTCAGGACTGTCC, downstream forward: _UP4_TAATGTGAAAAAACTTGAAA
• BKK01010 ([gene|43CFA3477B4EEB4CE0B2DF63ED2B5EDB171E79C7|nusG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTATTCAGGACTGTCC, downstream forward: _UP4_TAATGTGAAAAAACTTGAAA

References

Reviews

• 24632072

Original publications

• 18852477,16707701,11101662,10027981,23033921,21040729,20384694,26742846,19500594,29887376,31719185,32817529